Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid >=98.0% (KT) | 60-00-4 | MFCD00003541 | 50G
Ethylenediaminetetraacetic acid >=98.0% (KT) | Purity: >=98.0% (KT) | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 50G
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid anhydrous, free-flowing, powder, Redi-Dri(TM), ACS reagent, 99.4-100.6% | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS | 6976-37-0 | MFCD00002853 | 100g
BIS-TRIS | Purity: ≥99.0% (NT) | MW:209.24 | 6976-37-0 | MFCD00002853 | 100g
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt | 103404-87-1 | | 25g
HEPES hemisodium salt | Purity: 99% | MW: 249.3 | 103404-87-1 | 25g
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
Sigma Aldrich Fine Chemicals Biosciences CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | 1135-40-6 | MFCD00003837 | 1KG
CAPS anhydrous, free-flowing, Redi-Dri(TM), >=99% | Purity: >=99% | Mol Wt: 221.32 | 1135-40-6 | MFCD00003837 | 1KG
Sigma Aldrich Fine Chemicals Biosciences Tricine | 5704-04-1 | MFCD00004277 | 100 g
Tricine | Purity: ≥99% | Mol Wt: 179.17 | 5704-04-1 | MFCD00004277 | 100 g
Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | 60-00-4 | MFCD00003541 | 500G
Ethylenediaminetetraacetic acid purified grade, >=98.5%, powder | Purity: >=98.5% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 500G
Sigma Aldrich Fine Chemicals Biosciences CHES BioXtra, >=99.0% (titration) | 103-47-9 | MFCD00003835 | 100G
CHES BioXtra, >=99.0% (titration) | Purity: >=99.0% (titration) | Mol Wt: 207.29 | 103-47-9 | MFCD00003835 | 100G
Bioworld TT - SDS Running Buffer Kit (10X), 500 mL
TT-SDS Running Buffer 10X KitEach kit contains both a cathode and anode buffer (Catalog #: 765540 and 765542)
Bioworld PBS-Casein, 0.5%, 500 mL
Supplied in PBS as 0.5% casein ready for use.pH range 7.4 (+/- 0.1)Sold as 2 x 100mL or 500mL.
Bioworld PBS/Fish Gelatin (10X), 200 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)
Bioworld TT - SDS Running Buffer Kit (10X), 1 L
TT-SDS Running Buffer 10X KitEach kit contains both a cathode and anode buffer (Catalog #: 765540 and 765542)
Bioworld PBS/Fish Gelatin (10X), 500 mL
PBS/Fish Gelatin (1x) Phosphate Buffered Saline/fish gelatin 0.5% blocks, diluents, and coating solutions.Technical Information 1x: Fish Gelatin 0.5%PBS (1x)pH range: 7.4 (+/- 0.1)